Collagen, type I, alpha 1isthe major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage.
The COL1A1 gene produces a component of type I collagen, called the pro-alpha1(I) chain.
This chain combines with another pro-alpha1(I) chain and also with a pro-alpha2(I) chain (produced by the COL1A2 gene) to make a molecule of type I procollagen.
These triple-stranded, rope-like procollagen molecules must be processed by enzymes outside the cell.
Once these molecules are processed, they arrange themselves into long, thin fibrils that cross-link to one another in the spaces around cells.
The cross-links result in the formation of very strong mature type I collagen fibers.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human COL1a1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala1218-Ile1440 of human COL1a1 fused to His-Tag at N-terminal expressed in E. coli.
