TMEM4 encodes a predicted 182aa type II transmembrane protein.
The first 3 to 20aa of MSAP constitute the SA region, which is followed by a saposin -like domain interrupted by 2 stretches of 30 and 40aa Northern blot analysis detected MSAP expression in all adult and fetal tissues examined, with highest levels in adult placenta, liver, and pancreas.
Western blot analysis of placenta and brain detected MSAP at an apparent molecular mass of 21 kD.
Immunofluorescence microscopy showed colocalization of Msap with Mir in COS-7 cells.
In cultured rodent primary hippocampal neurons, the 2 proteins localized around the nucleus and extended into the neurites.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.