Calponin 2, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments.
The encoded protein could play a role in smooth muscle contraction and cell adhesion.
Two transcript variants encoding different isoforms have been found for this gene.
The CNN2 protein is 94.8% identical to mouse calponin h2, indicating that these proteins are homologs.
The predicted CNN2 protein has 309aa and a pI of 7.1.
It contains motifs that are present in CNN1 and CNN3: 3 tandem repeats of 29aa, an actin-binding domain, a VAV-homologous region, and 2 consensus phosphorylation sites for tyrosine kinase at the C terminus.
The 3-prime untranslated region of the CNN2 mRNA contains an Alu repetitive sequence in the antisense direction.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CNN2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Tyr269 of human CNN2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
