Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta.
It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin.
It is able to inhibit the ATPase activity of myosin and is thought to play a role in smooth muscle contraction.
These are the products of separate loci.
Northern blots of mouse uterus RNA indicated that h1 transcripts are about 10-fold higher than those of the h2 gene
The 297aa CNN1 contains a motif that is tandemly repeated 3 times.
The N terminus of CNN1 is markedly basic and highly homologous to that of CNN3.
CNN1 has a predicted pI of 9.4.
Northern blot analysis of RNA from human tissues showed that CNN1 is specifically expressed in smooth muscle.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CNN1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Tyr268 of human CNN1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
