CNDP1 encodes a member of the M20 metalloprotease family.
The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase.
This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region.
The mouse and rat CN1 homologs do not have an N-terminal signal peptide and are expressed exclusively in kidney.
Western blot analysis detected multiple protein bands in extracts of plasma and cerebrospinal fluid and 2 bands in cortex and hippocampus.
Immunohistochemical analysis detected CN1 in the cytosol of pyramidal neurons in the hippocampus and in large and small neurons of the temporal cortex.
In subcortical white matter, CN1 was expressed by small cells, presumably glial cells, and neuronal fibers.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CNDP1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp332-His507 of human CNDP1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
