CMTM1 belongs to the chemokine-like factor gene superfamily, a novel family that is similar to the chemokine and the transmembrane 4 superfamilies of signaling molecules.
The protein encoded by this gene may play an important role in testicular development.
Alternatively spliced transcript variants encoding different isoforms have been identified.
The CKLFSF1 gene encodes at least 23 alternatively spliced isoforms.
One variant encodes a 169aa protein that has a CC chemokine motif and 4 transmembrane segments.
CKLFSF1 shares closest similarity with CKLF, CKLFSF2, and CKLFSF7.
The N terminus of CKLFSF1 shares similarity with CCL17, and the C terminus shares similarity with CCL22and fractalkine (CX3CL1).
Northern blot analysis detected a 1.14-kb transcript that was highly expressed in testis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CMTM1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ala286 of human CMTM1, fused to His-Tag and T7-tag at Nt-terminal expressed in E. coli.
