The deduced 955aa protein contains an N-terminal signal sequence, followed by 2 cadherin repeats, a C-terminal transmembrane domain, and a cytoplasmic C terminus
CLSTN2 shares 58%aa identity with CLSTN1 and 94% identity with its mouse homolog.
Northern blot analysis of human tissues detected a 5-kb transcript expressed exclusively in brain.
In situ hybridization showed nonuniform Clstn2 expression in the majority of adult mouse brain regions.
In cerebral cortex, Clstn2 was found in the somas and neuropil of all layers, with highest immunoreactivity in cortical layers 5 and 6.
Immunoelectron microscopy showed that Clstn2 was prominently expressed in postsynaptic specializations of asymmetric synapses.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CLSTN2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg31-Gln271 of human CLSTN2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
