By searching for sequences similar to mouse Nkcl, Fernandes et al.
(2000) identified an EST clone isolated from a testis cDNA library that contained the full-length coding sequence for CLECSF11, which they designated HECL.
The deduced 183aa protein has a calculated molecular mass of 20 kD and contains a C-type carbohydrate recognition domain (CRD) and 3 putative N-glycosylation sites, but no transmembrane domain or signal peptide.
HECL shares significant homology with members of the C-type lectin superfamily, and its CRD is mose similar to that of mouse Nkcl.
The 4 cysteines responsible for the C-type CRD fold are perfectly conserved between HECL and mouse Nkcl.
Immunolocalization of epitope-tagged HECL expressed in COS cells revealed a cytoplasmic localization.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CLEC4C.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu5-Ile183 of human CLEC4C expressed in E. coli.
