CLEC11A encodes a member of the C-type lectin superfamily.
The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells.
An alternative splice variant has been described but its biological nature has not been determined.
Sequence analysis revealed a 21aa signal peptide; a glutamate-rich N-terminal domain, which includes an arg-gly-asp triplet; a leucine zipper; and a C-terminal C-type lectin domain, which is known to be a carbohydrate recognition domain.
The authors found O-glycan attachment sites but no N-glycosylation sites.
Northern blot analysis detected a 1.4-kb transcript that was strongly expressed in T and B lymphoblastoid, weakly expressed in monocytoid cell lines, and not expressed in fibroblasts.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CLEC11A.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala22-Phe323 of human CLEC11A, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
