Members of the chemokine-like factor superfamily, including CKLFSF8, share similarity with both the chemokine and the transmembrane 4 superfamilies of signaling molecules
By screening databases for sequences that share homology with chemokine-like factor (CKLF), followed by RT-PCR of a tonsil cDNA library, Han et al.
(2003) cloned CKLFSF8.
The deduced 173aa protein has 4 transmembrane segments and shares about 39% sequence identity with TM4SF11.
Northern blot analysis detected a 1.18-kb transcript expressed at high levels in liver and pancreas.
Han et al.
(2003) also cloned mouse Cklfsf8, which shares more than 95% sequence similarity with CKLFSF8.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.