84-4411-73　［受注停止］CITED1 (Cbp/p300 Interacting Transactivator, With Glu/Asp Rich Carboxy Terminal Domain 1, MSG1, Melanocyte-specific protein 1) 200ul　362580

特徴

• MSG1 transcripts in pigmented human epidermal melanocytes but did not detect MSG1 expression in any of the examined depigmented or weakly pigmented human melanoma cells.
• The deduced 193aa human MSG1 protein shares 75% overall sequence identity with mouse Msg1.
• The C-terminal 54aa of these proteins are 96% identical and highly acidic, with 22% of the aa being either aspartic acid or glutamic acid; this region forms a predicted amphipathic alpha-helical structure.
• Immunoprecipitation studies using an antibody against Msg1 detected a 27-kD protein in human epidermal melanocyte lysates.
• Immunofluorescence cytochemistry localized MSG1 to the nucleus of normal human epidermal melanocytes and human melanoma cells.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MPTTSRPALD VKGGTSPAKE DANQEMSSVA YSNLAVKDRK AVAILHYPGV ASNGTKASGA PTSSSGSPIG SPTTTPPTKP PSFNLHPAPH LLASMHLQKL NSQYQGMAAA TPGQPGEAGP LQNWDFGAQA GGAESLSPSA GAQSPAIIDS DPVDEEVLMS LVVELGLDRA NELPELWLGQ NEFDFTADFP SSC
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.