Cold stress induces in microorganisms the synthesis of several proteins that are involved in various cellular processes such as transcription, translation, and recombination.
The cold-inducible RNA-binding protein (Cirp) is induced in rodent cells by mild cold stress (32 degrees C).
Cirp consists of an N-terminal RNA-binding domain and a C-terminal gly-rich domain, and plays an essential role in cold-induced suppression of cell proliferation
CIRBP mRNA was constitutively expressed in all cell lines examined.
In all of the cell lines, the levels of CIRBP mRNA and protein were increased within 12 hours after a temperature downshift from 37 degrees C to 32 degrees C.
These results demonstrated that CIRBP is a cold-shock protein in human cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CIRBP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Glu172 of human CIRBP, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
