DNA fragmentation factor (DFF) induces the fragmentation of DNA associated with apoptosis.
A novel family of cell death-inducing DFF45-like effectors (CIDEs), including CIDEC, can also promote apoptosis.
The deduced 238aa protein has a predicted molecular mass of 26.8 kD and shares 44% and 38% identity with CIDEA and CIDEB, respectively.
Northern blot analysis detected a 1.3-kb transcript mainly in small intestine, heart, colon, and stomach, with lower expression observed in brain, kidney, and liver.
A 1.1-kb transcript was detected in placenta.
The authors identified a 164aa CIDEC isoform, which they called CIDE3-alpha, lacking the first N-terminal 74aa.
Immunofluorescence analysis localized CIDEC in a punctate manner to the cytosol.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat CIDEC.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Gln221 of rat CIDEC, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
