By genomic Southern analysis of hamster/human somatic cell hybrid DNAs, Anand and Lindstrom mapped the gene encoding the beta-3 subunit of the human neuronal nicotinic acetylcholine receptor to chromosome 8.
They indicated that the location of the corresponding gene in the mouse genome was not known.
Koyama et al.
used an exon amplification method to construct a transcriptional map of human chromosome 8.
With this method, transcribed sequences from defined regions of genomic DNA could be efficiently isolated using cosmid clones mapped to chromosome 8.
Sequence analysis revealed identity of a particular exon amplification fragment to the CHRNB3 gene.
This transcribed fragment was isolated from a cosmid clone that had previously been mapped to 8p11.2 by fluorescence in situ hybridization.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CHRNb3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu119-Arg230 of human CHRNb3, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
