Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy.
The CHRNB2 gene comprises 6 exons and spans approximately 9 kb of genomic DNA from the ATG start codon to the TGA stop codon.
Exon 1 contains the 5-prime untranslated region, the ATG start codon, and parts of the sequence coding for the putative signal peptide.
The hydrophobic transmembrane domains I through III are located in the large exon 5.
Exon 6 contains the transmembrane domain IV as well as the translation stop codon and the 3-prime untranslated region.
The CHRNB2 introns vary in size from 150 bp (intron 2) to 4.5 kb (intron 5).
The splicing at the junctions of CHRNB2 introns 2, 3, and 5 occurred between codons (splicing type 0).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CHRNb2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Trp24-Val70 and Glu86-Pro244 of human CHRNb2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
