(1996) cloned CHRNA2 from a thalamus cDNA library following low-stringency screening with the rat alpha-4-1 probe.
The deduced 503aa protein has a calculated molecular mass of 56.9kD and contains a signal peptide, 3 N-glycosylation sites, and 4 transmembrane regions.
The human and rat proteins share 84% sequence identity.
Human evolution is characterized by a dramatic increase in brain size and complexity.
To probe its genetic basis, Dorus et al.
(2004) examined the evolution of genes involved in diverse aspects of nervous system biology.
These genes, including CHRNA2, displayed significantly higher rates of protein evolution in primates than in rodents.
This trend was most pronounced for the subset of genes implicated in nervous system development.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CHRNa2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gln28-Leu241 of mouse CHRNa2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
