The deduced 429aa human protein shares 73% homology with the 426aa mouse protein.
CHL2 contains an N-terminal signal peptide, followed by 3 chordin (CHRD)-like cysteine-rich repeats (CR1 through CR3) and a C-terminal tail.
Northern blot analysis detected high expression of a 2.0-kb CHL2 transcript in adult human uterus, with lower expression in colon, heart, prostate, stomach, and skeletal muscle.
Expression was weak in placenta, testis, small intestine, trachea, and bone marrow, and no expression was detected in liver or kidney.
In situ hybridization of mouse embryos showed that Chl2 expression was restricted to chondrocytes of developing joint cartilage.
In adult mice, Chl2 expression was detected in connective tissues of reproductive organs, with highest expression in uterine myometrium.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CHRDL2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Arg26-Ser169 of mouse CHRDL2 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
