84-4411-53　［受注停止］CHRD (Chordin) 200ul　362560

特徴

• Chordin is a polypeptide that dorsalizes the developing embryo by binding ventralizing TGFbeta proteins such as bone morphogenetic proteins.
• It may also play a role in organogenesis.
• There are five named isoforms of this protein that are produced by alternative splicing.In humans, the chordin peptide is encoded by the CHRD gene.
• 948aa protein containing 4 internal cysteine-rich repeats and 4 potential N-glycosylation sites.
• Northern blot analysis detected high levels of a 3.7-kb Chrd transcript in 7-day postcoitum mouse embryos; its level decreased at later developmental stages and in adult tissues.
• Dot blot analysis of mRNA expression in human adult and fetal tissues showed that CHRD is expressed at the highest level in liver.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- R DPNTCFFEGQ QRPHGARWAP NYDPLCSLCT CQRRTVICDP VVCPPPSCPH PVQAPDQCCP VCPEKQDVRD LPGLPRSRDP GEGCYFDGDR SWRAAGTRWH PVVPPFGLIK CAVCTCKGGT GEVHCEKVQC PRLACAQPVR VNPTDCCKQC PVGSGAHPQL GDPMQADGPR GCRFAGQWFP ESQSWHPSVP PFGEMSCITC RCGAGVPHCE RDDCSLPL
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.