Calcium binding protein P22 is a phosphoprotein that binds to the sodium-potassium exchangers (NHEs).
This protein serves as an essential cofactor which supports the physiological activity of NHE family members.
It has protein sequence similarity to calcineurin B and it is also known to be an endogenous inhibitor of calcineurin activity
A partial sequence obtained from this screen was used to clone a novel full-length cDNA, designated CHP, from a second B-cell library.
The deduced 195aa protein contains 2 distinct and 2 potential EF-hand Ca(2+)-binding motifs and shares 43% and 33% identity with calcineurin B and calmodulin, respectively.
It also shares 99% sequence identity with the rat homolog.
Northern blot analysis demonstrated expression of a 4-kb CHP transcript in all 8 fetal tissues examined.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CHP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly2-His195 of human CHP, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
