CHL1 is a member of the L1 gene family of neural cell adhesion molecules.
It is a neural recognition molecule that may be involved in signal transduction pathways.
The deletion of one copy of this gene may be responsible for mental defects in patients with 3p- syndrome.
Several alternatively spliced transcript variants of this gene have been described, but their full length nature is not known.
The predicted CALL protein contains 1,224aa and has a calculated molecular mass of 136 kD.
It has a signal peptide, followed by 6 immunoglobulin (Ig) domains, 4 fibronectintype III (FNIII) domains, a single-pass transmembrane peptide, and a 105aa cytoplasmic domain.
The cytoplasmic domain of CALL is conserved among other members of the L1 subfamily and features sequence motifs that may involve CALL in signal transduction pathways.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CHL1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro35-Glu328 of human CHL1 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
