Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients.
The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture
Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression.
This enzyme may play a role in the degradation of chitin-containing pathogens.
The findings suggested that a single gene encodes the different isoforms of chitotriosidase.
The authors postulated that the enzyme may be involved in defense against and in degradation of chitin-containing pathogens such as fungi, nematodes, and insects.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CHIT1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met210-Ser464 of mouse CHIT1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
