Human articular chondrocytes secrete several proteins involved in cartilage biogenesis.
Hu et al.
(1996) identified YKL39 as a protein in conditioned medium from human articular cartilage chondrocytes in primary culture that copurified with YKL40 (HC gp39).
The name 'YKL' refers to the N-terminal aa sequence of the protein.
Hu et al.
(1996) found that YKL39 is most closely related by sequence analysis to YKL40.
Unlike YKL40, the YKL39 protein is not glycosylated; like YKL40, it is related to bacterial chitinases but does not have chitinase activity because it lacks the active site glutamate.
The authors found the highest level of YKL39 mRNA expression in chondrocytes, followed by synoviocytes, lung, and heart.
No YKL39 mRNA was detected in brain, spleen, pancreas, or liver.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CHI3L2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Tyr27-Tyr326 of human CHI3L2 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
