Chemerin is a novel protein identified as the natural ligand of ChemR23 (chemerinR), a previously orphan G protein-coupled receptor expressed in immature dendritic cells and macrophages.
It is a member of the Cystatin superfamily.
Chemerin is synthesized as a 163aa secreted precursor, prochemerin, which contains a hydrophobic 20 aa N-terminal sequence, an intervening 137aa Cystatin fold-containing domain, and a six aa C-terminal prosegment.
It is activated by proteases of the coagulation, fibrinolytic, and inflammatory cascades and converted into a full agonist of chemerinR by proteolytic removal of the last six aa.
Chemerin is a chemoattractant for cells bearing the Chem R23 receptor, including plasmacytoid dendritic cells and tissue macrophages.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CHEM.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Glu21-Phe155 of mouse CHEM fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
