Choline acetyltransferase (EC 2.3.1.6; abbreviated "ChAT") is an enzyme that is synthesized within the body of a neuron.
It is then transferred to the nerve terminal via axoplasmic flow.
The role of choline acetyltransferase is to join Acetyl-CoA to choline, resulting in the formation of the neurotransmitter acetylcholine.
Cholinergic systems are implicated in numerous neurologic functions.
Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease.
The protein encoded by this gene synthesizes the neurotransmitter acetylcholine.
Alternative splice variants have been found that contain alternative 5' untranslated exons.
Three of the four described splice variants encode identical 69kD proteins while one variant encodes both the 69kD and a larger 82kD protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ChAT.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp517-Pro732 of human ChAT fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
