CHAF1B corresponds to the p60 subunit and is required for chromatin assembly after replication.
The encoded protein is differentially phosphorylated in a cell cycle-dependent manner.
In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm.
This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
The predicted 559aa protein belongs to the WD (tryptophan-aspartate), or transducin, family.
These proteins are generally characterized by 4 to 8 copies of a repeat of approximately 40 residues that often ends with a WD sequence (p60 has 7 copies of the motif).
The p60 subunit also has a PEST (proline-glutamic acid-serine-threonine) box at its C terminus that is associated with proteins of short half-lives.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CHAF1B.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Asn199 of mouse CHAF1B, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
