(1983) determined that the 7 CGB genes are extremely similar but not identical.
Otani et al.
(1988) found that CGB3 and CBG8 were expressed at 2-fold lower levels than CGB5 following transfection into a mouse adrenocortical cell line.
Bo and Boime (1992) determined that most of the sequence variation among the CGB genes occurs in the nontranslated region of exon 1.
They further found that CGB3 was expressed at about the same levels at CGB8 in first trimester placenta and in a choriocarcinoma cell line, and both were expressed at a lower level than CGB5.
Jameson and Lindell (1988) determined that each of the CGB genes contains 3 exons.
Otani et al.
(1988) determined that the promoter regions contain no CAAT or TATAA boxes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CGb.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro31-Gln165 of human CGb, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
