Complement factor B is a a component of the alternative pathway of complement activation.
Factor B circulates in the blood as a single chain polypeptide.
Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb.
The active subunit Bb is a serine protease that associates with C3b to form the alternative pathway C3 convertase.
Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.
This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.
This cluster includes several genes involved in regulation of the immune reaction.
The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat CFB.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Val34-Asp159 of rat CFB, fused to two N-terminal Tags, His-Tag and MBP-tag, expressed in E. coli.
