CENTA2 contains an N-terminal ARF-GAP -like zinc finger motif, followed by 2 pleckstrin homology (PH) domains.
Northern blot analysis detected highest expression in placenta, spleen, kidney, skeletal muscle, and adrenal gland, with weaker expression in thyroid, liver, heart, lung, small intestine, and peripheral blood leukocytes.
CENTA2 was localized exclusively at the plasma membrane of transfected embryonic kidney cells.
CENTA2 expressed in insect cells bound inositol 1,3,4,5-tetrakisphosphate and phosphatidylinositol 3,4,5-trisphosphate with similar affinities.
Modeling studies suggested that there is only 1 class of binding sites, and stoichiometric analysis indicated that there are 2 binding sites per CENTA2 molecule.
Inhibition of PI3K caused membrane-bound CENTA2 to dissociate into the cytosol.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CENTa2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Cys49-Ser361 of human CENTa2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
