Cysteine dioxygenase (CDO) is a mammalian non-heme iron enzyme that catalyzes the conversion of L-cysteine to cysteine sulfinic acid (cysteine sulfinate) by incorporation of dioxygen.
The first step in the oxidation of cysteine to inorganic sulfate is its conversion to cysteine sulphinate by cysteine dioxygenase (CDO).
In rat, at least 2 enzymes catalyze this reaction, one cytosolic (CDO-I) and the other membrane-bound.
Rat liver CDO contains 1 atom of iron per molecule of protein, and the enzyme is activated by anaerobic incubation with either L-cysteine or its analogs.
The predicted 200aa human and rat proteins are 94% identical.
Northern blot analysis revealed that CDO-I is expressed as an approximately 1.5-kb mRNA in liver.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-1:400Immunocytochemistry: 1:50-1:500Immunohistochemistry (frozen): 1:50-1:500Immunohistochemistry (paraffin): 1:10-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CDO1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Asn200 of mouse CDO1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
