CDKN3 is an enzyme belongs to the dual specificity protein phosphatase family.
It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase.
This gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers.
CDI1 is expressed at the G1 to S transition, and the protein forms stable complexes with CDK2.
In vitro, CDI1 removes phosphate from tyrosine residues in model substrates, but a mutant protein that bears a lesion in the putative active site cysteine does not.
Overexpression of wildtype CDI1 delays progression through the cell cycle in yeast and HeLa cells; delay is dependent on CDI1 phosphatase activity.
The gene is also symbolized CDKN3 for cyclin-dependent kinase inhibitor 3.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-1:400Immunocytochemistry: 1:50-1:500Immunohistochemistry (frozen): 1:50-1:500Immunohistochemistry (paraffin): 1:10-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CDKN3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Arg212 of human CDKN3, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
