CD164 is a transmembrane isoform of the mucin-like glycoprotein MGC-24.
MGC-24 is a 24kD protein rich in hydroxyl aa and cysteine, and lack repeating motifs identified originally in human gastric carcinoma cells as a protein binding peanut agglutinin.
High levels of MGC-24 mRNA are expressed in normal tissues of the colon, small intestine and thyroid, and in some nude mouse-transplanted colorectal and pancreatic adenocarcinomas
Kurosawa et al (1999) have reported that in the mouse only the transmembrane form is expressed in many organs.
CD164 is expressed by human CD34(+) hematopoietic progenitor cells.
The subpopulations of CD34(+) cells expressing CD164 include the majority of CFU-GM, BFU-E, and hierarchically more primitive precursors (pre-CFU).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes mouse CD164.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Mo
Immunogen:Recombinant protein corresponding to Gln24-Asp162 of mouse CD164 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.