A coiled coil is a structural motif in proteins, in which 2-7 alpha-helices are coiled together like the strands of a rope (dimers and trimers are the most common types).
Many coiled coil type proteins are involved in important biological functions such as the regulation of gene expression e.g.
transcription factors.
Notable examples are the oncoproteins c-fos and jun, and the muscle protein tropomyosin.
The possibility of coiled coils for alpha-keratin was proposed by Francis Crick in 1952 as well as mathematical methods for determining their structure.
Remarkably, this was soon after the structure of the alpha helix was suggested in 1951 by Linus Pauling and coworkers.
Coiled coils usually contain a repeated pattern, hxxhcxc, of hydrophobic (h) and charged (c) aa residues, referred to as a heptad repeat.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CCDC60.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala388-Arg520 of human CCDC60 fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
