Precerebellin, or CBLN1, is the precursor of a conserved brain-specific hexadecapeptide termed cerebellin.
CBLN1 and other members of the precerebellin family also contain a C-terminal C1q signature domain that mediates trimeric assembly of atypical collagen complexes.
The deduced 193aa protein contains a hydrophobic N-terminal followed by the cerebellin sequence.
It also contains 3 potential N-glycosylation sites.
Over about 145aa in its C-terminal, CBLN1 shares 31.3% identity with C1QB, but it lacks N-terminal collagen-like motifs.
The cerebellin peptide is flanked by val-arg and glu-pro residues, suggesting that it is not liberated from precerebellin by the classical dibasic amino acid proteolytic mechanism seen in many neuropeptide precursors.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CBLN1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln22-Leu193 of human CBLN1 fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
