This enzyme is a member of a family of evolutionarily conserved cysteine protease proteins known as caspases.
Many of these enzymes are part of a proteolytic cascade that plays a central role in cell death by apoptosis.
Caspase-11 has been isolated and characterized as ICH-3 [ICE and CED3 homolog-3].
Caspase-11 gene expression in response to bacterial lipopolysaccharides and IFN-gamma requires NF-kappa-B and the signal transducer STAT1
By performing a yeast 2-hybrid assay to identify proteins that interact with CTD, Tanner et al.
(1997) isolated partial cDNAs encoding SIP1, which they called CTD-associated SR protein 11 (CASP11) or SR-related protein of 129kD (SRrp129).
Northern blot analysis detected expression of the approximately 6-kb SRrp129mRNA in all tissues tested.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CASP11.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg944-Trp1148 of human CASP11 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
