84-4409-92　CARNS1 (Carnosine Synthase 1, ATPGD1, ATP-Grasp Domain Containing 1) 200ul　362401

特徴

• CARNS1, a member of the ATP-grasp family of ATPases, catalyzes the formation of carnosine and homocarnosine, which are found mainly in skeletal muscle and the central nervous system, respectively.
• The deduced proteins contain 957aa and 950aa, respectively, and both contain 2 ATP-grasp domains, with highest conservation in the C-terminal catalytic domain.
• Gel filtration analysis showed that mouse Atpgd1, like the chicken enzyme, was expressed as a homotetramer of about 430kD in transfected HEK293 cells.Mouse and human ATPGD1 catalyzed the same reaction following expression in HEK293 cells.
• All 3 enzymes were not specific with respect to the aa serving as the acceptor, although L-histidine was preferred, and all 3 were also more efficient in synthesizing carnosine than homocarnosine and other dipeptides.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.5-2ug/mlWestern Blot: 5-20ug/ml Immunohistochemistry (Paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.