Calpain small subunit 1 is a protein encoded by the CAPNS1 gene.
Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases.
Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress.
Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes.
This gene encodes a small subunit common to both calpain I and II and is associated with myotonic dystrophy.
Two transcript variants encoding the same protein have been identified for this gene.Regulatory subunit of the calcium-regulated non- lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CAPNS1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ser2681 of human CAPNS1 fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
