Calpain (calcium-dependent protease) is an intracellular protease that requires calcium for its catalytic activity.
Two isozymes, calpain I (mu-calpain) and calpain II (m-calpain), with different calcium requirements, have been identified.
Both are heterodimers composed of L (large, catalytic, 80kD) and S (small, regulatory, 30kD) subunits.
The isozymes share an identical S subunit (CAPNS1), with the differences arising from the L subunits, L1 (CAPN1) and L2 (CAPN2).
The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins.
The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit.
Calpain-1 catalytic subunit is the large subunit of the ubiquitous enzyme, calpain 1.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CAPN1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Lys426-Val676 of human CAPN1 fused to His-Tag at N-terminal expressed in E. coli
