Calmodulin Like Protein 5 is a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins.
Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so
Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes.
In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes.Particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation.
Very low expression in lung.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CALML5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Glu146 of human CALML5 fused to His-Tag and T7-tag at N-terminal expressed in E. coli
