CALML3, the deduced 148aa protein contains 4 helix-loop-helix Ca(2+)-binding motifs and shares 85% identity with vertebrate calmodulin.
Northern blot analysis detected no expression in fibroblast, teratoma, or erythroleukemia cell lines.
CLP was expressed exclusively in the epithelium of the tissues surveyed and was most abundant in thyroid, breast, prostate, kidney, and skin.
CLP expression appeared to increase in stratified epithelium during differentiation, as illustrated in skin, where CLP staining intensified from the basal through the spinous to the granular layers.
Significant differences between the electric surface potentials at the target protein-binding regions of CLP and calmodulin suggested that the ranges of CLP and calmodulin target proteins do not fully overlap.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CALML3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Lys149 of human CALML3 fused to His-Tag and T7-tag at N-terminal expressed in E. coli
