Calcyclin-binding protein is a calcyclin binding protein.
It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins.
It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin.
The full-length SIP cDNA encodes a predicted 228aa protein.
Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8aa difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80aa protein, SIP-short (SIPS).
SIPS arises by alternative splicing.
The full-length human SIP protein shares 93% aa identity with the mouse Sip protein, 40% identity with a counterpart in Drosophila, and 20% identity with the human SGT1 protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CACYBP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala2-Phe228 of human CACYBP fused to His-Tag and T7-tag at N-terminal expressed in E. coli
