84-4409-50　C9 (Complement Component 9) 200ul　362359

特徴

• Activation of the complement system results in formation of the membrane attack complex (MAC) on the membranes of target cells.
• The complex is assembled by sequential addition of 1 molecule each of C5b, C6, C7, and C8 and 6 to 16 molecules of the ninth component, C9
• DiScipio et al.
• (1984) screened a human liver cDNA library by the colony-hybridization technique using 2 radiolabelled oligonucleotide probes that correspond to known regions of the C9 aa sequence.
• The cDNA coding for C9 was sequenced and the protein sequence--537aa in a single polypeptide chain-was derived.
• The N-terminal half of C9 is predominantly hydrophilic and the C-terminal half is more hydrophobic.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- PRTPC RDRVAEESEL GLTAGYGINI LGMEPLRTPF DNEFYNGLCD RVRDEKTYYR KPWNVVSLIY ETKADKSFRT ENYDEHLEVF KAINREKTSN FNADFALKFS ATEVPEKGAG EVSPAEHSSK PTNISAKFKF SYFMGKNFRR LSSYFSQSKK MFVHLRGVVQ LGRFVMRNRD VVLRSTFLDD VKALPTSYEK GEYFGFLETY GTHYSTSGSL GGQYEIVYVL DKASMKEKGV DLNDVKHCLG FNMDLRIPLQ DDLKDASVTA SVNADGCIKT DNGKTVNITR DNIIDDVISF IRGGTREQAI LLKEKILRGD KTFDKTDFAN WASSLANAPA LISQRMSPIY NLIPLKIKDA YIKKQNLEKA VEDYIDEFST KR
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.