C7 is a single-chain plasma glycoprotein involved in the cytolytic phase of complement activation (Hobart et al.,1995).
Mutations in the C7 gene cause C7 deficiency, a defect associated with increased susceptibility to neisserial recurrent infections (Barroso et al., 2004).
Hobart et al.
(1978) identified 3 structural forms of C7, concluded they are the products of 3 codominant alleles at an autosomal locus, and found that the C6 and C7 loci are closely linked to each other but not to the HLA complex.
By Southern blot analysis of hybrid cell DNAs using cDNA probes, Jeremiah et al.
demonstrated that the human C6 and C7 genes are located on chromosome 5.
See Coto et al.
(1991) for linkage information indicating that C6, C7, and C9 are closely situated; C9 was mapped to 5p13.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat C7.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Glu1-Ser14 of rat C7 fused to His-Tag and T7-tag at N-terminal expressed in E. coli
