C6 is 1 of 5 late-acting proteins of complement that participate in assembly and function of the MAC (DiScipio and Hugli, 1989).
The deduced C6 protein contains 934aa, including a 21aa N-terminal signal sequence and 2 N-glycosylation sites.
The mature 913aa C6 protein shares 29% aa identity with C7, and 55 of 56 cysteines in C7 match those in C6.
The C6 polypeptide chain is cross-linked by 32 disulfide bonds, and most cysteines are located in short, discrete segments that exhibit homology with a variety of proteins, including thrombospondin, low density lipoprotein receptor(LDLR), epidermal growth factor (EGF), and complement factors H (CFH) and I (CFI ).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human C6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln682-Ala934 of human C6, fused to His-Tag and T7-tag at N-terminal expressed in E. coli
