C5a is a protein fragment released from complement component C5.
In humans, the polypeptide contains 74aa.
NMR spectroscopy proved that the molecule is composed of four helices and loops connecting the helices.
On the N terminal a short 1.5 turn helix is also present.
The longest helix -IV- develops three disulfide bonds with helix II and III.
C5a is rapidly metabolised by a serum enzyme, carboxypeptidase B to a 73aa form, C5a des-Arg
C5a binds to a receptor protein on the surface of target cells, C5aR or CD88.
This is a member of the G-protein-coupled receptor superfamily of proteins, predicted to have seven transmembrane helical domains of largely hydrophobic aa residues, forming three intra-and three extra-cellular loops, with an extracellular N-terminal and an intracellular C-terminal.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human C5a.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Thr678-Arg751 of human C5a, fused to His-Tag and GST-tag at N-terminal expressed in E. coli
