C4b-binding protein is involved in regulation of the complement system.
It is a multimeric protein comprising 7 identical alpha chains, encoded by C4BPA, and a single beta chain, encoded by C4BPB.
The alpha and beta chains have molecular masses of 70 and 45kD, respectively.
Both subunits belong to a superfamily of proteins composed predominantly of tandemly arranged short consensus repeats (SCR) of approximately 60aa in length (Aso et al., 1991).
Brodeur et al.
(2003) identified a 23kD protein that bound CD40 as C4BP-alpha.
Flow cytometric analysis demonstrated binding of C4BP to human B-cell lines expressing CD40, but not to cells from CD40-deficient patients.Aso et al.
(1991) found that the C4BPA gene comprises 12 exons and spans about 40 kb.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse C4BPa.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Lys57-Pro306 of mouse C4BPa, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
