Complement component 4 is a protein involved in the complement system.It is cleaved into proteins 4a and 4b.
C4b forms part of C3-convertase, in conjunction with 2a:C4b can bind CR1.
C4b is the large fragment formed when Complement C4 is cleaved by Complement C1S.
The membrane-bound C4b binds Complement C2A, a SERINE PROTEASE, to form C4b2a (CLASSICAL PATHWAY C3 CONVERTASE) and subsequent C4b2a3b (CLASSICAL PATHWAY C5 CONVERTASE).
In 3 black-American patients with SLE, Wilson and Perez (1988) found complete deficiency of plasma C4B.
In a 9-year-old girl with SLE and complete C4 deficiency, Welch et al.
(1990) found uniparental isodisomy 6.
The girl had 2 identical chromosome 6 haplotypes from the father and none from the mother.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- N VNFQKAINEK LGQYASPTAK RCCQDGVTRL PMMRSCEQRA ARVQQPDCRE PFLSCCQFAE SLRKKSRDKG QAGLQR
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human C4b.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asn680-Arg756 of human C4b, fused to His-Tag and T7-tag at N-terminal expressed in E. coli
