There are two forms of C3-convertase (EC 3.4.21.43).
The first is an enzyme composed of the C4b-C2a complex, which forms during the Classical pathway of the complement system.
It is formed when C1s cleaves off C2b from a membrane-bound C4b-C2a complex.
The second consists of the C3bBb complex, which forms during the alternative complement pathway.
It is formed when membrane bound C3b binds to factor B which is in turn cleaved by factor D into Ba and Bb.
Bb remains bound to C3b while Ba is released into the surrounding medium
Soluble C3-convertase, also known as C4b2a, catalyzes the proteolytic cleavage of C3 into C3a and C3b as part of the classical complement system as well as the mannan-binding lectin pathway.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry: 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt..
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin, 50% glycerol.
Specificity：Recognizes mouse C3c. Species Crossreactivity: human.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to mouse C3c expressed in E. coli.
