Complement C3 is the major plasma proteins of the immune system complement pathways.
The synthesis of these proteins is increased in response to inflammation and infection but at a slower rate than for traditional acute phase proteins.
Complement protein C3 is the fourth component to attach in the complement reaction sequence.
It is a beta-globulin with a sedimentation coefficient of 5.5 and a molecular weight of 185,000.
C3 is a central molecule in the complement system whose activation is essential for all the important functions performed by this system.
C3 can promote phagocytosis during an inflammatory response against pathogens, but unregulated activation of C3 could lead to host cell damage.
Low level of serum C3 associates with hypocomplementaemia, primary biliary cirrhosis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes porcine C3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Po
Immunogen：Recombinant protein corresponding to Asp23-Thr300 of porcine C3, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
