84-4409-27 [受注停止]C1QTNF1 (C1q And Tumor Necrosis Factor Related Protein 1, GIP, CTRP1, ZSIG37, G Protein Coupled Receptor Interacting Protein) 200ul 362336
84-4409-27 [受注停止]C1QTNF1 (C1q And Tumor Necrosis Factor Related Protein 1, GIP, CTRP1, ZSIG37, G Protein Coupled Receptor Interacting Protein) 200ul 362336
The extracellular N-terminal contains a TNF family cysteine-rich region, followed by a transmembrane domain, a C-terminal collagen-like sequence of 14 Gly-X-Y repeats, and a globular domain.
Northern blot analysis detected prominent expression in heart and lower expression in placenta, liver, skeletal muscle, kidney, prostate and ovary.
Little to no expression was detected in other tissues examined
In transfected HEK293 and COS cells, epitope-tagged GIP was expressed in vesicular structures surrounding the nucleus and near the cell surface.
Western blot analysis detected epitope-tagged GIP at about 40kD and 130kD, suggesting GIP can form trimers.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes human C1QTNF1.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to Arg26-Pro281 of human C1QTNF1 fused to His-Tag and GST-tag at N-terminal expressed in E. coli.