Reid (1974) reported a partial aa sequence of 95 residues of the 191 residues in the oxidized A chain of human subcomponent C1q.
This region of the A chain contains a repeating sequence of glycine-X-Y, where X is often proline and Y is often hydroxyproline, for 78 residues.
The 5 hydroxylysine residues and 5 hydroxyproline residues in the oxidized A chain are all in these 78 residues and only in the Y position of the repeating sequence.Prolonged collagenase digestion of the oxidized A chain yielded a large, apparently C-terminal peptide containing most of the noncollagenous sequences present in the chain.
Reid (1974) concluded that the A chain of C1q contains a collagen-like region that constitutes most of the N-terminal half of the chain.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human C1qA.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala28-A la245 of human C1qA fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
